Cleaning involves removing debris from the surface to allow for a disinfectant to come into direct contact with pathogens. Disinfecting involves destroying pathogens by the use of chemical compounds. First responders and producers can use the following to guide their cleaning and disinfecting protocols after response to an emergency on a livestock operation.
Cleaning is the first step in the process and can be done by completing the following steps:
- Dry brush to remove matter. This step is extremely important and there should be minimal debris left on the equipment following dry cleaning.
- After dry cleaning is completed, wet cleaning should be done. This is completed by using a detergent-water mixture and scrubbing thoroughly followed by a thorough rinse.
- Allow all surfaces to dry completely and follow with disinfection.
Disinfection is meant to destroy pathogens. There are a variety of disinfectants with a range of effectiveness for different microorganisms. Some common ones include household bleach, oxidizing agents and betadine. These are effective in destroying the majority of pathogens. Always read the label for mixing instructions, contact time, required PPE and surfaces the product is safe to use on. When disinfectants are applied, they must remain wet and in contact with the entire surface for the duration of the prescribed contact time. For more information on types of disinfectants to use for different pathogens, view the table below.
| |
Household Bleach
(5.25%) |
Oxidizing Agents: Accelerated Hydrogen Peroxide (Accel/Rescue, Intervention, Oxivir Tb, Prevail), Potassium Peroxymonosulfate (Virkon) |
Betadine
(10%) |
Recommended Dilution Rate
(Disinfectant: Water) |
1:32 for general disinfection
1:10 for disease outbreak |
Check product label as concentrations differ |
1:10 |
| Recommended Contact Time |
1 minute |
1-5 minutes |
1 minute |
| |
++ = very effective; + = effective; +/- = variable or limited activity; - = not effective |
Mycoplasmas
Mycoplasma spp.: M. bovis (bovine respiratory disease or BRD, pneumonia, mastitis, arthritis), M. dispar (chronic respiratory infection), M. canadense (respiratory disease, arthritis, joint infections) |
++ |
++ |
+ |
Enveloped viruses
Bovine coronavirus, bovine viral diarrhea (BVD) 1&2, malignant catarrhal fever, bovine herpes virus 1,2&3 (infectious bovine rhinotracheitis, IBR), cowpox virus, pseudocowpox virus (milker’s nodules), bovine leukemia virus (BLV) |
+ |
+ |
+ |
Gram-positive bacteria
Bovine tuberculosis, Clostridium spp.: C. chauvoei (blackleg), C. septicum (malignant edema), C. perfringens (enterotoxemia), C. tetani (tetanus); Streptococcus spp.: S. aureus (mastitis); Arcanobacterium pyogenes & Trueperella pyogenes (abscess formation and infections in uterus, joints, liver and lungs) |
+ |
+ |
++ |
Gram-negative bacteria
E. coli (bovine colibacillosis, enterotoxemia), Salmonella spp. (fever, diarrhea, abortion, death), BRD (shipping fever), Histophilus somni (brain inflammation, pneumonia, death), Brucellosis, Campylobacter spp. (abortion) |
+ |
+ |
++ |
Vegetative fungi
Candida spp. (oral or vaginal infections, mastitis). |
+ |
+ |
+A |
Fungal spores
Ringworm |
+ |
+/- |
+/- |
Non-enveloped viruses
Foot and mouth disease (FMD), bovine adenoviruses A, B&C, bovine papillomavirus |
+ |
+/- |
- |
Mycobacteria
Johne’s disease, bovine tuberculosis |
+ |
+/- |
- |
Protozoal oocysts (parasites)
Cryptosporidium, Coccidiosis |
- |
- |
- |
Bacterial endospores
Anthrax |
+ |
+ |
- |
Prions
BSE, CWD |
-B |
-B |
- |
(The Center for Food Security & Public Health, Iowa State University, 2025)
A. Generally, not effective against Pseudomonas (chronic mastitis)
B. High concentrations may be effective against prion diseases